| 게재년월 |
2021/09 |
| 논문제목 |
Generation of a more efficient prime editor 2 by addition of the Rad51 DNA-binding domain
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| 참여교수 |
김형범
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| 학술지명 |
NATURE COMMUNICATIONS |
| 초록 |
Although prime editing is a promising genome editing method, the efficiency of prime editor 2 (PE2) is often insufficient. Here we generate a more efficient variant of PE2, named hyPE2, by adding the Rad51 DNA-binding domain. When tested at endogenous sites, hyPE2 shows a median of 1.5- or 1.4- fold (range, 0.99- to 2.6-fold) higher efficiencies than PE2; furthermore, at sites where PE2-induced prime editing is very inefficient (efficiency < 1%), hyPE2 enables prime editing with efficiencies ranging from 1.1% to 2.9% at up to 34% of target sequences, potentially facilitating prime editing applications. |
| 게재정보 |
Nat Commun. 2021 Sep 23;12(1):5617 |
| 총저자명 |
Myungjae Song, Jung Min Lim, Seonwoo Min, Jeong-Seok Oh, Dong Young Kim, Jae-Sung Woo, Hiroshi Nishimasu, Sung-Rae Cho, Sungroh Yoon, Hyongbum Henry Kim
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